ABSTRACT
OBJECTIVE To establish the method for t he content determination of 5 active components in Yixin badiran jibuya granules and their fingerprints. METHODS High performance liquid chromatography method was adopted to determine the contents of luteolin- 7-O-β-D-glucuronide（LG），apigenin-7-O-glucuronide（APG），rosmarinic acid （RA），diosmetin-7-O-β-D-glucuronide （DG）and tilianin （TL）in 10 batches（No. S 1-S10）of Yixin badiran jibuya granules. The fingerprints of 10 batches of Yixin badiran jibuya granules were drawn by Similarity Evaluation System of Chromatographic Fingerprint of Traditional Chinese Medicine（2004 A edition ），and similarity evaluation and cluster analysis were also performed. RESULTS The contents of LG ， APG，RA，DG and TL in 10 batches of Yixin badiran jibuya granules were 0.279 5-0.449 9，0.082 4-0.135 3，0.184 8-0.472 1， 0.149 0-0.332 6，0.311 2-0.623 3 mg/g，respectively. A total of 13 common peaks were demarcated in the fingerprints and were identified as LG （peak 2）APG（peak 6），RA（peak 7），DG（peak 8），TL（peak 11）. The similarity ranged from 0.598 to 0.990. The results of cluster analysis showed that S 6-S10 were clustered into one category and S 1-S5 were clustered into one category. CONCLUSIONS Established method for content determination and fingerprints can be used for the quality control for Yixin badiran jibuya granules.